Caracterización toxicológica en renacuajos de la rana (gastrotheca riobambae) en función de las concentraciones de n- fosfonometilglicina
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
PUCE - Ibarra
Abstract
El N-fosfonometilglicina (glifosato) es un herbicida ampliamente utilizado en la agricultura para el control de malezas, pero su uso continuo y excesivo ha generado problemas de toxicidad en ecosistemas acuáticos – terrestres por su estabilidad química, como es la biodegradación y biocumulación en la cadena trófica. En el caso de anfibios cumplen un rol muy fundamental ya que son el grupo de vertebrados considerados excelentes indicadores ambientales por tener un ciclo de vida, acuático –terrestre, además de desarrollar un papel importante en el ecosistema como depredador o presa para ayudar a mantener el equilibrio de la naturaleza.
El presente estudio consistió en caracterizar suelos de actividad agrícola de la parroquia La Esperanza y analizar la presencia de N-fosfonometilglicina por técnicas de HPLC, obteniendo resultados de 0,1mg.kg-1 en suelo removido para siembra de maíz, 0,0 mg.kg-1 en suelo de cultivo de maíz en cosecha y 0,4mg.kg-1 en suelo de cultivo de cebada, además evaluar la dosis letal en renacuajos de la rana marsupial andina Gastrotheca riobambae con la finalidad de dar a conocer su importancia como bioindicador.
El ensayo toxicológico refleja diferentes comportamientos en renacuajos de la Gastrotheca riobambae tales como agresividad, periodo de inactividad y finalmente la muerte, la DL50 fue 7487,26mg.l-1 y la DL100 fue 9791,44mg.l-1 con una mortalidad total de la población.
N-phosphonomethylglycine (glyphosate) is a herbicide widely used in agriculture for the control of weeds, but its continuous and excessive use has generated problems of toxicity in aquatic - terrestrial ecosystems due to its chemical stability, such as biodegradation and biocumulation in the food chain. In the case of amphibians they play a very fundamental role since they are the group of vertebrates considered excellent environmental indicators for having a life cycle, aquatic -terrestrial, in addition to developing an important role in the ecosystem as a predator or prey to help maintain the balance of nature. The present study consisted in characterizing agricultural activity soils of the La Esperanza parish and analyzing the presence of N-phosphonomethylglycine by HPLC techniques, obtaining results of 0.1mg.kg-1 in soil removed for maize sowing, 0.0 mg .kg-1 in maize crop soil in harvest and 0.4mg.kg-1 in barley culture soil, in addition to evaluate the lethal dose in tadpoles of the Andean marsupial frog Gastrotheca riobambae with the purpose of publicizing its importance as a bioindicator. The toxicological test reflects different behaviors in tadpoles of the Gastrotheca riobambae such as aggressiveness, inactivity period and finally death, the LD50 was 7487.26mg.l-1 and the DL100 was 9791.44mg.l-1 with a total mortality of the population
N-phosphonomethylglycine (glyphosate) is a herbicide widely used in agriculture for the control of weeds, but its continuous and excessive use has generated problems of toxicity in aquatic - terrestrial ecosystems due to its chemical stability, such as biodegradation and biocumulation in the food chain. In the case of amphibians they play a very fundamental role since they are the group of vertebrates considered excellent environmental indicators for having a life cycle, aquatic -terrestrial, in addition to developing an important role in the ecosystem as a predator or prey to help maintain the balance of nature. The present study consisted in characterizing agricultural activity soils of the La Esperanza parish and analyzing the presence of N-phosphonomethylglycine by HPLC techniques, obtaining results of 0.1mg.kg-1 in soil removed for maize sowing, 0.0 mg .kg-1 in maize crop soil in harvest and 0.4mg.kg-1 in barley culture soil, in addition to evaluate the lethal dose in tadpoles of the Andean marsupial frog Gastrotheca riobambae with the purpose of publicizing its importance as a bioindicator. The toxicological test reflects different behaviors in tadpoles of the Gastrotheca riobambae such as aggressiveness, inactivity period and finally death, the LD50 was 7487.26mg.l-1 and the DL100 was 9791.44mg.l-1 with a total mortality of the population
Description
Keywords
N, Fosfonometilglicina, Toxicidad, Anuros