CIENCIAS AGRÍCOLAS Y AMBIENTALES

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Browsing CIENCIAS AGRÍCOLAS Y AMBIENTALES by Author "Arcos Ortiz, César Arturo"

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    Aislamiento de consorcio bacteriano de la fuente termal de Pucará, perteneciente al cantón Urcuquí - Imbabura para la lixiviación de cobre del proyecto tipo A "Biosensores"
    (Pontificia Universidad Católica del Ecuador Sede Ibarra, 2019-07) Arcos Ortiz, César Arturo
    The objective of the research was to isolate a native strain from the thermal waters of Pucará, Urcuquí, Imbabura, capable of leaching copper without the need to use conventional methods, for which three sampling points were established in the thermal water sources of the study area: main reservoir (P1), secondary reservoir (P2) and slope attached to the river (P3), of which the respective physico-chemical analysis was carried out, having as main results a pH of 5.9, a temperature of 40 ° C and a copper concentration of 3.2 mg.l-1, which served as experimental base for its replication in the laboratory. In the experimental phase, the bacterial consortiums were isolated, using Petri dishes with LB agar, replicating the conditions of the medium, to characterize the existing strains by their size, shape and color, where 36 different strains were found which were transferred to tubes of LB Invitrogen broth assay under extreme conditions of 100 mg.l-1 copper, a pH of 3.3 and the same temperature, where 7 copper-resistant strains could be found, identified by DNA extraction and the polymerase chain reaction, sending to Macrogen for its respective sequencing and identification. Copper concentrations were measured at 24, 48 and 72 hours by acid digestion of water for the total of recoverable or dissolved metals for analysis by spectroscopy, with an optimum retention time of 48 hours in which two strains leached a maximum of 81% (Thermothelomyces thermophilus) and 78% (Spirochaeta thermophila).