Pioneer study of population genetics of Rhodnius ecuadoriensis (Hemiptera: Reduviidae) from the central coastand southern Andean regions of Ecuador

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dc.careerEscuela de Ciencias Biológicases
dc.category.authorprincipalen_US
dc.contributor.authorGrijalva Cobo, Mario Javier
dc.contributor.authorVillacis Salazar, Anita Gabriela
dc.contributor.authorYumiseva Marín, César Alberto
dc.contributor.correspondingGrijalva Cobo, Mario Javier
dc.countryEcuadores
dc.date.accessioned2023-11-04T21:31:12Z
dc.date.available2023-11-04T21:31:12Z
dc.date.issued2017-09
dc.dedication.authorTCes
dc.description.abstractEffective control of Chagas disease vector populations requires a good understanding of the epidemiological components, including a reliable analysis of the genetic structure of vector populations. Rhodnius ecuadoriensis is the most widespread vector of Chagas disease in Ecuador, occupying domestic, peridomestic and sylvatic habitats. It is widely distributed in the central coast and southern highlands regions of Ecuador, two very different regions in terms of bio-geographical characteristics. To evaluate the genetic relationship among R. ecuadoriensis populations in these two regions, we analyzed genetic variability at two microsatellite loci for 326 specimens (n = 122 in Manabí and n = 204 in Loja) and the mitochondrial cytochrome b gene (Cyt b) sequences for 174 individuals collected in the two provinces (n = 73 and = 101 in Manabí and Loja respectively). The individual samples were grouped in populations according to their community of origin. A few populations presented positive FIS, possible due to Wahlund effect. Significant pairwise differentiation was detected between populations within each province for both genetic markers, and the isolation by distance model was significant for these populations. Microsatellite markers showed significant genetic differentiation between the populations of the two provinces. The partial sequences of the Cyt b gene (578 bp) identified a total of 34 haplotypes among 174 specimens sequenced, which translated into high haplotype diversity (Hd = 0.929). The haplotype distribution differed among provinces (significant Fisher's exact test). Overall, the genetic differentiation of R. ecuadoriensis between provinces detected in this study is consistent with the biological and phenotypic differences previously observed between Manabí and Loja populations. The current phylogenetic analysis evidenced the monophyly of the populations of R. ecuadoriensis within the R. pallescens species complex; R. pallescens and R. colombiensis were more closely related than they were to R. ecuadoriensis.en_US
dc.facultyCiencias Exactas y Naturaleses
dc.id.author1708757742
dc.id.author1706504535
dc.id.author1709331571
dc.id.type1
dc.identifier.doihttps://doi.org/10.1016/j.meegid.2017.05.019
dc.identifier.issn1567-1348
dc.identifier.urihttps://repositorio.puce.edu.ec/handle/123456789/4786
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S156713481730179X?via%3Dihub
dc.indexed.databaseScimago Journal Rankes
dc.language.isoen
dc.list.authorsVillacís, A., Marcet, P., Yumiseva, C., Dotson, E., Tibayrenc, M., Brenière, S., & Grijalva, M.
dc.magazine.pageRange116-127
dc.magazine.titleInfection, Genetics and Evolutionen_US
dc.magazine.volumeChapter53
dc.rightsOpenAccessen
dc.statepublisheden_US
dc.subjectGenética de poblacioneses
dc.subjectEnfermedad de Chagases
dc.subjectGenética de poblaciones
dc.subjectEnfermedad de Chagas
dc.titlePioneer study of population genetics of Rhodnius ecuadoriensis (Hemiptera: Reduviidae) from the central coastand southern Andean regions of Ecuadoren_US
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