Identification of a fungal 1,8-cineole synthase from Hypoxylon sp. with specificity determinants in common with the plant synthases

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dc.careerCiencias Biológicasen_US
dc.category.authorprincipalen_US
dc.contributor.authorNarváez Trujillo, Elizabeth Alexandra
dc.contributor.authorPortero Pico, Carolina Elizabeth
dc.contributor.correspondingNarváez Trujillo, Elizabeth Alexandraen_US
dc.countryEcuadoren_US
dc.date.accessioned2023-11-04T21:38:55Z
dc.date.available2023-11-04T21:38:55Z
dc.date.issued03/02/2015
dc.dedication.authorTCen_US
dc.description.abstractTerpenes are an important and diverse class of secondary metabolites widely produced by fungi. Volatile compound screening of a fungal endophyte collection revealed a number of isolates in the family Xylariaceae, producing a series of terpene molecules including 1,8-cineole. This compound is a commercially important component of eucalyptus oil used in pharmaceutical applications and has been explored as a potential biofuel additive. The genes that produce terpene molecules such as 1,8-cineole have been little explored in fungi, providing an opportunity to explore the biosynthetic origin of these compounds. Through genome sequencing of cineole producing isolate E7406B we were able to identify eleven new terpene synthase genes. Expressing a subset of these genes in E. coli allowed identification of the hyp3 gene, responsible for 1,8-cineole biosynthesis, the first monoterpene synthase discovered in fungi. In a striking example of convergent evolution, mutational analysis of this terpene synthase revealed an active site asparagine critical for water capture and specificity during cineole synthesis, the same mechanism used in an unrelated plant homologue. These studies have provided insight into the evolutionary relationship of fungal terpene synthases to those in plants and bacteria, and further established fungi as a relatively untapped source of this important and diverse class of compounds.en_US
dc.facultyCiencias Exactas y Naturalesen_US
dc.id.author1000620672en_US
dc.id.author1716265309
dc.id.type1en_US
dc.identifier.doi10.1074/jbc.M114.636159en_US
dc.identifier.issn1083-351X
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/25648891en_US
dc.identifier.urihttps://repositorio.puce.edu.ec/handle/123456789/5713
dc.indexed.databaseScimago Journal Ranken_US
dc.list.authorsShaw, J., Berbasova., P., Narváez, A. & Strobel, et al.en_US
dc.magazine.pageRange8511-8526en_US
dc.magazine.titleJournal of Biological Chemistryen_US
dc.magazine.volumeChapter290en_US
dc.rightsClosedAccessen_US
dc.statepublisheden_US
dc.subjectEnzimologíaen_US
dc.subjectProteínas microbianasen_US
dc.subjectHongosen_US
dc.titleIdentification of a fungal 1,8-cineole synthase from Hypoxylon sp. with specificity determinants in common with the plant synthasesen_US
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