Development of Peptide-Based Lineage-Specific Serology for Chronic Chagas Disease: Geographical and Clinical Distribution of Epitope Recognition.

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dc.careerCiencias Biológicases
dc.category.authorvisitoren_US
dc.contributor.authorGrijalva Cobo, Mario Javier
dc.contributor.correspondingBhattacharyya, Tapan.
dc.countryEcuadores
dc.date.accessioned2023-11-04T21:35:45Z
dc.date.available2023-11-04T21:35:45Z
dc.date.issued2014
dc.dedication.authorTPes
dc.description.abstractChagas disease, caused by infection with the protozoan Trypanosoma cruzi, remains a serious public health issue in Latin America. Genetically diverse, the species is sub-divided into six lineages, known as Tcl-TcVI, which have disparate geographical and ecological distributions. TcII, TcV, and TcVI are associated with severe human disease in the Southern Cone countries, whereas TcI is associated with cardiomyopathy north of the Amazon. T. cruzi persists as a chronic infection, with cardiac and/or gastrointestinal symptoms developing years or decades after initial infection. Identifying an individual´s history of T. cruzi lineage infection directly by genotyping of the parasite is complicated by the low parasitaemia and sequestration in the host tissues. We have appllied here serology against lineage-specific epitopes of the T. cruzi surface antigen TSSA, as an indirect approach to allow identification of infecting lineage. Chagasic sera from chronic patients from a range of endemic countries were tested by ELISA against synthetic peptides representing lineage-specific TSSA epitopes bound to avidin-coated ELISA plates via a biotin labelled polyethylene glycol-glycine spacer to increase rotation and ensure each amino acid side chain could freely interact with their antibodies. 79/113 (70%) of samples from Brazil, Bolivia, and Argentina recognised the TSSA epitope common to lineages TcII/TcV/TcVI. Comparison with clinical information showed that a higher proportion of Brazilian TSSApep-II/V/VI responders had ECG abnormalities than non-responders (38% vs 17%; p<0.0001). Among northern chagasic sera 4/20 (20%) from Ecuador reacted with this peptide; 1/12 Venezuelan and 1/34 Colombian samples reacted with TSSApep-IV. In addition, a proposed TcI-specific epitope, described elsewhere, was demonstrated herre to be highly conserved across lineages and therefore not applicable to lineage-specific serology. These resultas demonstrate the considerable potential for synthetic peptide serology to investigate the infection history of individuals, geographical and clinical associations of T. cruzi lineagesen_US
dc.facultyCiencias Exactas y Naturaleses
dc.id.author1708757742
dc.id.type1
dc.identifier.doi10.1371/journal.pntd.0002892
dc.identifier.urihttps://repositorio.puce.edu.ec/handle/123456789/5454
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pntd.0002892
dc.indexed.databaseScimago Journal Rankes
dc.language.isoen
dc.list.authorsBhattacharyya, T., Falconar, A., Luquetti, A., Costales, J.A., Grijalva, M.J., Lewis, M.D., Messenger, L.A., Tran, T., Ramirez, J.D., Guhl, F., et al.
dc.magazine.pageRange01-12
dc.magazine.titlePlos Oneen_US
dc.magazine.volumeChapter8
dc.rightsClosedAccessen
dc.statepublisheden_US
dc.subjectDevelopmentes
dc.subjectPeptidees
dc.subjectChagas diseasees
dc.subjectGeographical and clinical distributiones
dc.subjectDevelopment
dc.subjectPeptide
dc.subjectChagas disease
dc.subjectGeographical and clinical distribution
dc.titleDevelopment of Peptide-Based Lineage-Specific Serology for Chronic Chagas Disease: Geographical and Clinical Distribution of Epitope Recognition.en_US
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