Evaluación de la reproducción in vitro de la especie Tillandsia emergens mediante explantes con el fin del desarrollo de una metodología de multiplicación para la PUCESI
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Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
PUCE - Ibarra
Abstract
La especie Tillandsia emergens Mez y Sodiro es una planta endémica del Ecuador
perteneciente a la familia de las bromelias. Esta especie actualmente se encuentra en estado
vulnerable debido a los desastres naturales (incendios forestales) y la acción antrópica del
hombre (tala indiscriminada) en el país; por esta razón se consideró realizar una propagación
in vitro para la conservación de esta especie. Por lo cual se realizó diferentes transectos que
determinaron cuál fue la incidencia de esta especie en 4 comunidades de la Parroquia de San
Roque, Provincia de Imbabura. Además, se desarrolló un protocolo de cultivo in vitro a partir
de hojas jóvenes, yemas y semillas; estos fueron sembrados en un medio de cultivo preparado
de Murashige y Skoog a su totalidad de concentración (4,3g.l-1), sacarosa (30g.l-1),
agar (10g.l-1), suplementados con antioxidantes ácido cítrico y ácido ascórbico (100mg.l-1),
además con hormonas reguladoras de crecimiento ANA y BAP (10 mg.l-1). Transcurrido los
90 días de la siembra in vitro se evalúo en cada uno de los tratamientos (hojas jóvenes, yemas
y semillas) la formación de brotes, la longitud y sobrevivencia del explante. Siendo el mejor
tratamiento el de las semillas en todas las variables, debido a que obtuvo los mejores
resultados en comparación con los demás explantes (yemas y hojas jóvenes).
The species Tillandsia emergens Mez y Sodiro is an endemic plant of Ecuador belonging to the family of bromeliads. This species is currently in a vulnerable state due to natural disasters (forest fires) and human action (indiscriminate felling) in the country; for this reason it was considered to carry out an in vitro propagation for the conservation of this species. Therefore, different transects were carried out that determined the incidence of this species in 4 communities of the Parroquia de San Roque, Province of Imbabura. In addition, an in vitro culture protocol was developed from young leaves, buds and seeds; These were planted in a culture medium prepared from Murashige and Skoog at full concentration (4.3g.l-1), sucrose (30g.l-1), agar (10g.l-1), supplemented with antioxidants citric acid and ascorbic acid (100 g.l-1), in addition with growth regulating hormones ANA and BAP (10mg.l-1). After 90 days of sowing in vitro, shoot formation, length and survival of the explant were evaluated in each of the treatments (young leaves, buds and seeds). The best treatment is that of the seeds in all the variables, because it obtained the best results in comparison with the other explants (buds and young leaves).
The species Tillandsia emergens Mez y Sodiro is an endemic plant of Ecuador belonging to the family of bromeliads. This species is currently in a vulnerable state due to natural disasters (forest fires) and human action (indiscriminate felling) in the country; for this reason it was considered to carry out an in vitro propagation for the conservation of this species. Therefore, different transects were carried out that determined the incidence of this species in 4 communities of the Parroquia de San Roque, Province of Imbabura. In addition, an in vitro culture protocol was developed from young leaves, buds and seeds; These were planted in a culture medium prepared from Murashige and Skoog at full concentration (4.3g.l-1), sucrose (30g.l-1), agar (10g.l-1), supplemented with antioxidants citric acid and ascorbic acid (100 g.l-1), in addition with growth regulating hormones ANA and BAP (10mg.l-1). After 90 days of sowing in vitro, shoot formation, length and survival of the explant were evaluated in each of the treatments (young leaves, buds and seeds). The best treatment is that of the seeds in all the variables, because it obtained the best results in comparison with the other explants (buds and young leaves).
Description
Keywords
Cultivo in vitro, Tillandsia emergens, Murashige y skoog