Potencial bactericida o bacteriostático del veneno de abejas (Apis mellifera) para microorganismos causantes de mastitis clínica en vacas lecheras
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
PUCE - Ibarra
Abstract
Esta investigación se relaciona con el control de mastitis clínica de vacas lecheras, producida por microrganismos como Staphylococcus aureus, Streptococcus agalactiae y Escherichia coli, para cuyo propósito se encontró en las propiedades del veneno de abejas (Apis mellifera) una alternativa al problema de productores lecheros en general. Se partió del muestreo de vacas clínicamente mastíticas, cultivos microbiológicos de los patógenos, se purificaron para su aislamiento y secuenciación molecular, con el auxilio de personal del laboratorio de Microbiología de la PUCE. Se buscaron concentraciones mínimas inhibitorias (CMI), con el uso de soluciones acuosas de apitoxina de 0,5 hasta 1,0 mg de apitoxina por litro de agua grado uno. La CMI fue administrada parenteralmente a vacas clínicamente confirmadas con mastitis, mediante exploración visual y determinación de unidades formadoras de colonias (UFC) y conteo de células somáticas (CCS). Las mejores CMIs fueron 0,9 mg.l-1 para Staphylococcus aureus y Streptococcus agalactiae, mientras que para Escherichia coli fue de 1,0 mg.l-1. Concluyéndose que la apitoxina como producto natural es un antibacteriano total para las bacterias objeto de este estudio, encontrándose en este recurso apícola una solución al problema número uno de los productores de leche de vacas.
This investigation is related to the control of clinical mastitis of dairy cows, produced by microorganisms such as Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli, for whose purpose was found in the properties of bee venom (Apis mellifera) an alternative to the problem of dairy producers in general. We started with the sampling of clinically mastitic cows, microbiological cultures of the pathogens, they were purified for their isolation and molecular sequencing, with the help of personnel from the Microbiology laboratory of the PUCE. Minimum inhibitory concentrations (MIC) were sought, with the use of aqueous solutions of apitoxin from 0.5 to 1.0 mg of apitoxin per liter of water grade one. The IMC was administered parenterally to clinically confirmed cows with mastitis, by visual examination and determination of colony forming units (CFU) and somatic cell count (SCC). The best MICs were 0.9 mg.l-1 for Staphylococcus aureus and Streptococcus agalactiae, while for Escherichia coli it was 1.0 mg.l-1. Concluding that apitoxin as a natural product is a total antibacterial for the bacteria that is the object of this study, being in this apicultural resource a solution to the number one problem of cow milk producers.
This investigation is related to the control of clinical mastitis of dairy cows, produced by microorganisms such as Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli, for whose purpose was found in the properties of bee venom (Apis mellifera) an alternative to the problem of dairy producers in general. We started with the sampling of clinically mastitic cows, microbiological cultures of the pathogens, they were purified for their isolation and molecular sequencing, with the help of personnel from the Microbiology laboratory of the PUCE. Minimum inhibitory concentrations (MIC) were sought, with the use of aqueous solutions of apitoxin from 0.5 to 1.0 mg of apitoxin per liter of water grade one. The IMC was administered parenterally to clinically confirmed cows with mastitis, by visual examination and determination of colony forming units (CFU) and somatic cell count (SCC). The best MICs were 0.9 mg.l-1 for Staphylococcus aureus and Streptococcus agalactiae, while for Escherichia coli it was 1.0 mg.l-1. Concluding that apitoxin as a natural product is a total antibacterial for the bacteria that is the object of this study, being in this apicultural resource a solution to the number one problem of cow milk producers.
Description
Keywords
Antibiogramas, Apitoxina, Staphylococcus aureus, Mastitis