Adaptation of the AFLP technique as a new tool to detect genetic instability and transposition in interspecific hybrids

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dc.careerBiologyen_US
dc.category.authorprincipalen_US
dc.contributor.authorVela Peralta, Doris Jimena
dc.contributor.correspondingVela, Doris.
dc.date.accessioned2023-11-04T21:41:46Z
dc.date.available2023-11-04T21:41:46Z
dc.date.issued2011
dc.dedication.authorTCes
dc.description.abstractAn adapted amplified fragment length polymorphism (AFLP) protocol is presented for detection of hybrid in- stability in the genome of interspecific hybrids between Drosophila buzzatii and D. koepferae species. Analyses of 15 AFLP instability markers (new bands detected in hybrids) show that up to 81% are the result of transposable element (TE) activity. Twenty TEs associated with AFLP instability markers have been detected by this method in backcross hybrids and segmental hybrids, demonstrating its validity in detecting transposition events occurring during the hybridization process. New insertions of Helena TE have been observed in the hybrid genome after hy- bridization of the TGTCG22 instability marker by FISH. The AFLP marker technique proved to be an efficient method that improves upon traditional and bioinformatic tools previously used to detect TE mobilization. This newly adapted AFLP protocol may also be applied to a large number of organisms outside the Drosophila genus, making it of interest to evolutionary and population genetic researchers working with species where the knowl- edge of the genome is scarce.en_US
dc.facultyBiologyes
dc.id.author-
dc.identifier.doi10.2144/000113655
dc.identifier.urihttps://repositorio.puce.edu.ec/handle/123456789/5902
dc.indexed.databaseOtheres
dc.language.isoen
dc.list.authorsVela, Doris., García-Guerreiro, María Pilar., Fontdevila, Antonio.
dc.magazine.pageRange247-250
dc.magazine.titleBioTechniquesen_US
dc.magazine.volumeChapter50
dc.rightsOpenAccessen
dc.statepublisheden_US
dc.titleAdaptation of the AFLP technique as a new tool to detect genetic instability and transposition in interspecific hybridsen_US
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