Development and evaluation of copper electrodes, modified with bimetallic nanoparticles, to be used as sensors of cysteine-rich peptides synthesized by tobacco cells exposed to cytotoxic levels of cadmium

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dc.careerEscuela de Ciencias Químicases
dc.category.authorprincipalen_US
dc.contributor.authorEspinoza Montero, Patricio Javier
dc.contributor.authorFernández Martínez, Lenys Mercedes
dc.contributor.correspondingEspinoza Montero, Patricio Javier
dc.countryEcuadores
dc.date.accessioned2023-11-04T21:48:52Z
dc.date.available2023-11-04T21:48:52Z
dc.date.issued2019-06
dc.dedication.authorTCes
dc.description.abstractWe report on two new electrochemical sensors which, coupled to differential pulse voltammetry, constitutes a useful tool for diagnosis of heavy metal pollution. The electrochemical sensors AgHgNf/Cu and the AgBiNf/Cu were obtained by deposition of bimetallic particles of AgHg or AgBi on copper electrodes covered with a Nafion (Nf) film, respectively. Micrographs of the electrode’s surface showed evenly scattered bimetallic particles, with an approximate diameter of 150 nm, embedded in the Nafion (Nf) film. In order to test the electrodes, the hydrogen evolution signal according to the Brdička reaction was measured for the determination of cysteine-rich peptides (CRp) produced by plants. To check the accuracy of the electrodes, real samples of Nicotiana tabacum cells exposed to cytotoxic levels of cadmium were tested. The AgHgNf/Cu electrode produced detection limits (DLs) of 0.088 µmol L−1 for Cysteine and 0.139µmol L−1 for Glutathione, while for the AgBiNf/Cu electrode DLs were 0.41 µmol L−1 for cysteine and 0.244 µmol L−1 for glutathione. Thus, the new electrodes could be a useful analytical electrochemical system very convenient for fieldwork. The electrodes were capable of direct, accurate, and sensitive detection of synthesized peptides, despite the complex matrix where the Nicotiana tabacum cells were grown.en_US
dc.facultyCiencias Exactas y Naturaleses
dc.id.author2100381306
dc.id.author1757845787
dc.id.type1
dc.identifier.doihttps://dx.doi.org/10.3390%2Fmolecules24122200
dc.identifier.issn1420-3049
dc.identifier.urihttps://repositorio.puce.edu.ec/handle/123456789/6262
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631066/
dc.indexed.databaseScimago Journal Rankes
dc.language.isoen
dc.list.authorsVelasco, C., Espinoza, P., Montero, M., Alvarado, J., Jadán, M., Carrera, P., Fernández, L.
dc.magazine.pageRange1-10
dc.magazine.titleMoleculeses
dc.magazine.volumeChapter24(12)
dc.rightsOpenAccessen
dc.statepublisheden_US
dc.subjectMetales pesadoses
dc.subjectElectroquímicaes
dc.subjectContaminación químicaes
dc.subjectMetales pesados
dc.subjectElectroquímica
dc.subjectContaminación química
dc.titleDevelopment and evaluation of copper electrodes, modified with bimetallic nanoparticles, to be used as sensors of cysteine-rich peptides synthesized by tobacco cells exposed to cytotoxic levels of cadmiumen_US
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