Evaluación de la actividad antifúngica del extracto total de una especie de la familia Ericaceae sobre el agente etiológico Neopestalotiopsis javaensis
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Date
2022
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Publisher
PUCE - Ibarra
Abstract
A nivel mundial el consumo de aguacate ha estado en una tendencia creciente en los últimos años, por lo que en el Ecuador se ha incrementado su producción; sin embargo, la calidad del producto se ha visto afectada mayoritariamente por la aparición de la enfermedad conocida como “roña”, con el fin de buscar alternativas para este problema, se ha hecho uso de extractos vegetales que presentan actividad antifúngica y a su vez, ofrezcan alternativas al uso de fungicidas sintéticos y reduzcan la contaminación ambiental. El principal objetivo de la presente investigación fue evaluar la actividad antifúngica del extracto total de la especie vegetal Pernettya prostrata perteneciente a la familia Ericaceae sobre el agente etiológico Neopestalotiopsis javaensis. Para cumplir con el objetivo planteado se recolectó la especie vegetal en la Laguna de Cuicocha, misma que fue sometida a un proceso de deshidratación para luego obtener el extracto total mediante el proceso de maceración dinámica usando metanol como solvente, se evaluaron cuatro concentraciones seriadas de 500, 250, 125 y 62.5 mg/ml siguiendo la metodología de Kirby-Bauer de difusión directa en agar sobre el agente etiológico previamente aislado Neopestalotiopsis javaensis. Se obtuvo un extracto total de Pernettya prostrata con un rendimiento del 31,48%, sin embargo, las cuatro concentraciones estudiadas del extracto total de Pernettya prostrata no mostraron actividad frente al agente etiológico en estudio.
Worldwide, avocado consumption has been on a growing trend in recent years, which is why its production has increased in Ecuador; however, the quality of the product has been affected mainly by the appearance of the disease known as scab. In order to find alternatives to this problem, plant extracts have been used that have antifungal activity and, in turn, offer alternatives to the use of synthetic fungicides and reduce environmental pollution. The main objective of this research was to evaluate the antifungal activity of the total extract of the plant species Pernettya prostrata belonging to the Ericaceae family on the etiological agent Neopestalotiopsis javaensis. In order to achieve the objective, the plant was collected in the Cuicocha Lake, which was subjected to a dehydration process to obtain the total extract through the dynamic maceration process using methanol as a solvent. It was evaluated at four serial concentrations of 500, 250, 125 and 62.5 mg/ml following the Kirby-Bauer methodology of direct diffusion in agar on the previously isolated etiological agent Neopestalotiopsis javaensis. A total extract of Pernettya prostrata was obtained with a yield of 31.48%; however, the four concentrations of the total extract of Pernettya prostrata did not show activity antifungal against the etiological agent under study.
Worldwide, avocado consumption has been on a growing trend in recent years, which is why its production has increased in Ecuador; however, the quality of the product has been affected mainly by the appearance of the disease known as scab. In order to find alternatives to this problem, plant extracts have been used that have antifungal activity and, in turn, offer alternatives to the use of synthetic fungicides and reduce environmental pollution. The main objective of this research was to evaluate the antifungal activity of the total extract of the plant species Pernettya prostrata belonging to the Ericaceae family on the etiological agent Neopestalotiopsis javaensis. In order to achieve the objective, the plant was collected in the Cuicocha Lake, which was subjected to a dehydration process to obtain the total extract through the dynamic maceration process using methanol as a solvent. It was evaluated at four serial concentrations of 500, 250, 125 and 62.5 mg/ml following the Kirby-Bauer methodology of direct diffusion in agar on the previously isolated etiological agent Neopestalotiopsis javaensis. A total extract of Pernettya prostrata was obtained with a yield of 31.48%; however, the four concentrations of the total extract of Pernettya prostrata did not show activity antifungal against the etiological agent under study.
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Keywords
Pernettya prostrata, Neopestalotiopsis javaensis, Extracto vegetal, Roña, Actividad antifúngica