Costales Cordero, Jaime AlfredoOcaña Mayorga, Sofía BeatrizVillacis Salazar, Anita GabrielaYumiseva Marín, César Alberto2023-11-042023-11-0420171935-2735https://doi.org/10.1371/journal.pntd.0005710https://repositorio.puce.edu.ec/handle/123456789/5964https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005710Rhodnius ecuadoriensis is the main triatomine vector of Chagas disease, American trypanosomiasis, in Southern Ecuador and Northern Peru. Genomic approaches and next generation sequencing technologies have become powerful tools for investigating population diversity and structure which is a key consideration for vector control. Here we assess the effectiveness of three different 2b restriction site-associated DNA (2b-RAD) genotyping strategies in R. ecuadoriensis to provide sufficient genomic resolution to tease apart microevolutionary processes and undertake some pilot population genomic analyses.enOpenAccessGenética molecularGenómicaTripanosomiasis americanaGenética molecularGenómicaTripanosomiasis americana