Montúfar Galárraga, Rommel JoseloRon Melo, Santiago Rafael2023-11-042023-11-042019-052168-04502168-0450https://doi.org/10.1002/aps3.1243https://repositorio.puce.edu.ec/handle/123456789/4927https://bsapubs.onlinelibrary.wiley.com/doi/10.1002/aps3.1243Premise: Third-generation sequencing methods generate significantly longer reads than those produced using alternative sequencing methods. This provides increased possibilities for the study of biodiversity, phylogeography, and population genetics. We developed a protocol for in-solution enrichment hybridization capture of long DNA fragments applicable to complete plastid genomes. Methods and results: The protocol uses cost-effective in-house probes developed via long-range PCR and was used in six non-model monocot species (Poaceae: African rice, pearl millet, fonio; and three palm species). DNA was extracted from fresh and silica gel-dried leaves. Our protocol successfully captured long-read plastome fragments (3151 bp median on average), with an enrichment rate ranging from 15% to 98%. DNA extracted from silica gel-dried leaves led to low-quality plastome assemblies when compared to DNA extracted from fresh tissue. Conclusions: Our protocol could also be generalized to capture long sequences from specific nuclear fragments.enOpenAccessBiodiversidadFilogenéticaHibridación de ADNBiodiversidadFilogenéticaHibridación de ADN