Diverse Escherichia coli lineages from domestic animals carrying colistin resistance gene mcr-1 in an Ecuadorian household

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dc.careerMedicinaes
dc.category.authorprincipales
dc.contributor.authorVillacís Acuña, José Eduardo
dc.contributor.correspondingVillacís Acuña, José Eduardoes
dc.countryEcuadores
dc.date.accessioned2023-11-04T21:50:24Z
dc.date.available2023-11-04T21:50:24Z
dc.date.issued2020
dc.dedication.authorTCes
dc.description.abstractObjective: The aim of this study was to detect potential animal reservoirs of Escherichia coli carrying the mcr-1 gene in an Ecuadorian household. Methods: The mobile colistin-resistance gene, mcr-1, was first detected in Ecuador in a commensal E. coli isolate from a boy. A cross-sectional study was performed to detect the possible source of colistin-resistant E. coli in the boy's household. Faecal swabs and soil faecal samples were collected from companion animals. Samples were plated on selective media to isolate colistin-resistant E. coli and isolates were submitted to PCR detection of mcr-1, pulsed field gel electrophoresis (PFGE), and multi-locus sequences typing (MLST). Moreover, the genomes of all the isolates were sequenced. Results: Three different colistin-resistant E. coli sequence types (ST3941, 1630 and 2170), corresponding to three PFGE patterns, were obtained from a chicken and two dogs; these isolates were different from the human isolate (ST609). By whole-genome sequencing, the mcr-1.1 gene was found on IncI2 plasmids with very high nucleotide identity. Conclusions: Our results indicate a polyclonal dissemination of mcr-1.1 in the environment surrounding the first MCR-producing E. coli strain reported in Ecuador. Our findings support the idea of lateral dissemination of mcr-1.1 gene between unrelated E. coli isolates.es
dc.facultyMedicinaes
dc.id.author1715477186es
dc.id.type1es
dc.identifier.doihttps://doi.org/10.1016/j.jgar.2019.12.002es
dc.identifier.issn2213-7173
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/31841712/es
dc.identifier.urihttps://repositorio.puce.edu.ec/handle/123456789/6338
dc.indexed.databaseOtheres
dc.list.authorsLoayza-Villa, F., Salinas, L., Tijet, N., Villavicencio, F., Tamayo, R., Salas, S., Rivera, R., Villacis, J., Satan, C., Ushiña, L., Muñoz, O., Zurita, J., Melano, R., Reyes, J. y Trueba, G.es
dc.magazine.pageRange63-67es
dc.magazine.titleJournal of Global Antimicrobial Resistancees
dc.magazine.volumeChapter22es
dc.rightsOpenAccesses
dc.statepublishedes
dc.subjectResistencia antimicrobianaes
dc.subjectResistencia a medicamentoses
dc.subjectAnimales de compañíaes
dc.subjectEscherichia colies
dc.titleDiverse Escherichia coli lineages from domestic animals carrying colistin resistance gene mcr-1 in an Ecuadorian householdes
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