High prevalence of B2-ST131 clonal group among extended-spectrum β-lactamase-producing Escherichia coli isolated from bloodstream infections in Quito, Ecuador

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dc.careerMedicinaes
dc.category.authorprincipalen_US
dc.contributor.authorZurita Salinas, Lucía Jeannete
dc.contributor.correspondingZurita Salinas, Lucía Jeannete
dc.countryEcuadores
dc.date.accessioned2023-11-04T21:47:32Z
dc.date.available2023-11-04T21:47:32Z
dc.date.issued2019-12
dc.dedication.authorTCes
dc.description.abstractObjectives: The purpose of this study was to describe the clonal relationships and phylogroups of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-Ec) isolated from patients with bacteraemia in three hospitals in Quito, Ecuador. Methods: Between June 2013 and September 2014, a total of 4354 blood cultures were performed in three hospitals located in different areas of Quito. A BACTECTM system was used for blood culture, and the VITEK®2 system was used for species identification and in vitro antimicrobial susceptibility testing. The ESBL genotype, presence of the blaCTX-M, blaTEM and blaSHV genes, and the phylogenetic group of E. coli isolates was determined by PCR. Clonal groups were established by multilocus sequence typing (MLST). Results: Of 929 blood cultures positive for Gram-negative bacilli, 181 (19.5%) were positive for E. coli, representing the most frequent bacteraemia isolates in each hospital. Of the 181 E. coli isolates, 57 (31.5%) were ESBL-Ec. The main sources of ESBL-Ec bacteraemia were urinary tract infection (40; 70.2%), biliary tract infection (10; 17.5%) and other infections (7; 12.3%). The majority of ESBL-Ec isolates (39; 68.4%) from the three hospitals belonged to the virulent phylogenetic group B2, of which 36/39 (92.3%) were ST131 and 33/36 (91.7%) carried the blaCTX-M-15 gene. Conclusion: These results provide knowledge of the phylogenetic relationships of E. coli from bacteraemia in Ecuadorian patients. ST131 has emerged in ESBL-Ec, representing an important public-health problem because this multiresistant clone is considered to be a vehicle for the propagation of antimicrobial resistance genes and is a highly virulent, well-adapted human pathogen.en_US
dc.facultyMedicinaes
dc.id.author1703079119
dc.id.type1
dc.identifier.doihttps://doi.org/10.1016/j.jgar.2019.04.019
dc.identifier.issn2213-7165
dc.identifier.issn2213-7173
dc.identifier.urihttps://repositorio.puce.edu.ec/handle/123456789/6192
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/31077859/
dc.indexed.databaseOtheres
dc.language.isoen
dc.list.authorsZurita, J., Solís, M., Ortega, D., Barba, P., Paz Y Miño, A., & Sevillano, G.
dc.magazine.pageRange216-221
dc.magazine.titleJournal of Global Antimicrobial Resistanceen_US
dc.magazine.volumeChapter19
dc.rightsOpenAccessen
dc.statepublisheden_US
dc.subjectBacteriases
dc.subjectEscherichia colies
dc.subjectEnfermedades infecciosases
dc.subjectBacterias
dc.subjectEscherichia coli
dc.subjectEnfermedades infecciosas
dc.titleHigh prevalence of B2-ST131 clonal group among extended-spectrum β-lactamase-producing Escherichia coli isolated from bloodstream infections in Quito, Ecuadoren_US
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