Determination of Antioxidant Activity by Oxygen Radical Absorbance Capacity (ORAC-FL), Cellular Antioxidant Activity (CAA), Electrochemical and Microbiological Analyses of Silver Nanoparticles Using the Aqueous Leaf Extract of Solanum mammosum L

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dc.careerEscuela de Ciencias Químicases
dc.category.authorprincipales
dc.contributor.authorEspinoza Montero, Patricio Javier
dc.contributor.authorFernández Martínez, Lenys Mercedes
dc.contributor.authorMeneses Olmedo, Lorena Maribel
dc.contributor.authorPilaquinga Flores, María Fernanda
dc.contributor.correspondingEspinoza Montero, Patricio Javieres
dc.countryEcuadores
dc.date.accessioned2023-11-04T21:30:21Z
dc.date.available2023-11-04T21:30:21Z
dc.date.issued2021
dc.dedication.authorTCes
dc.description.abstractPurpose: The importance of studying polyphenolic compounds as natural antioxidants has encouraged the search for new methods of analysis that are quick and simple. The synthesis of silver nanoparticles (AgNPs) using plant extracts has been presented as an alternative to determine the total polyphenolic content and its antioxidant activity. Methods: In this study, aqueous leaf extract of Solanum mammosum, a species of plant endemic to South America, was used to produce AgNPs. The technique of oxygen radical absorption capacity using fluorescein (ORAC-FL) was used to measure antioxidant activity. The oxidation of the 2´,7´-dichlorodihydrofluorescein diacetate (DCFH2-DA) as fluorescent probe was used to measure cellular antioxidant activity (CAA). Electrochemical behavior was also examined using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Total polyphenolic content (TPH) was analyzed using the Folin-Ciocalteu method, and the major polyphenolic compound was analyzed by high performance liquid chromatography with diode array detector (HPLC/DAD). Finally, a microbial analysis was conducted using Escherichia coli and Bacillus sp. Results: The average size of nanoparticles was 5.2 ± 2.3 nm measured by high-resolution transmission electron microscopy (HR-TEM). The antioxidant activity measured by ORAC-FL in the extract and nanoparticles were 3944 ± 112 and 637.5 ± 14.8 µM ET/g of sample, respectively. Cellular antioxidant activity was 14.7 ± 0.2 for the aqueous extract and 12.5 ± 0.2 for the nanoparticles. The electrochemical index (EI) was 402 μA/V for the extract and 324 μA/V for the nanoparticles. Total polyphenolic content was 826.6 ± 20.9 and 139.7 ± 20.9 mg EGA/100 g of sample. Gallic acid was the main polyphenolic compound present in the leaf extract. Microbiological analysis revealed that although leaf extract was not toxic for Escherichia coli and Bacillus sp., minor toxic activity for AgNPs was detected for both strains. Conclusion: It is concluded that the aqueous extract of the leaves of S. mammosum contains nontoxic antioxidant compounds capable of producing AgNPs. The methods using AgNPs can be used as a fast analytical tool to monitor the presence of water-soluble polyphenolic compounds from plant origin. Analysis and detection of new antioxidants from plant extracts may be potentially applicable in biomedicine.es
dc.facultyCiencias Exactas y Naturaleses
dc.id.author2100381306es
dc.id.author1757845787es
dc.id.author1002429411es
dc.id.author1715307110es
dc.id.type1es
dc.identifier.doihttps://doi.org/10.2147/IJN.S302935es
dc.identifier.issn1178-2013
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/34471354/es
dc.identifier.urihttps://repositorio.puce.edu.ec/handle/123456789/4448
dc.indexed.databaseScimago Journal Rankes
dc.list.authorsPilaquinga, F., Morey, J., Fernandez, L., Espinoza-Montero, P., Moncada-Basualto, M., Pozo-Martinez, J., Olea-Azar, C., Bosch, R., Meneses, L., Debut, A., & Piña, M.es
dc.magazine.pageRange5879-589es
dc.magazine.titleInternational Journal of Nanomedicinees
dc.magazine.volumeChapter16es
dc.rightsOpenAccesses
dc.statepublishedes
dc.subjectVoltametríaes
dc.subjectNanopartículases
dc.subjectAntioxidanteses
dc.titleDetermination of Antioxidant Activity by Oxygen Radical Absorbance Capacity (ORAC-FL), Cellular Antioxidant Activity (CAA), Electrochemical and Microbiological Analyses of Silver Nanoparticles Using the Aqueous Leaf Extract of Solanum mammosum Les
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