Long-fragment targeted capture for long-read sequencing of plastomes
| dc.career | Escuela de Ciencias Biológicas | es |
| dc.category.author | principal | en_US |
| dc.contributor.author | Montúfar Galárraga, Rommel Joselo | |
| dc.contributor.author | Ron Melo, Santiago Rafael | |
| dc.contributor.corresponding | Montúfar Galárraga, Rommel Joselo | |
| dc.country | Ecuador | es |
| dc.date.accessioned | 2023-11-04T21:31:52Z | |
| dc.date.available | 2023-11-04T21:31:52Z | |
| dc.date.issued | 2019-05 | |
| dc.dedication.author | TC | es |
| dc.description.abstract | Premise: Third-generation sequencing methods generate significantly longer reads than those produced using alternative sequencing methods. This provides increased possibilities for the study of biodiversity, phylogeography, and population genetics. We developed a protocol for in-solution enrichment hybridization capture of long DNA fragments applicable to complete plastid genomes. Methods and results: The protocol uses cost-effective in-house probes developed via long-range PCR and was used in six non-model monocot species (Poaceae: African rice, pearl millet, fonio; and three palm species). DNA was extracted from fresh and silica gel-dried leaves. Our protocol successfully captured long-read plastome fragments (3151 bp median on average), with an enrichment rate ranging from 15% to 98%. DNA extracted from silica gel-dried leaves led to low-quality plastome assemblies when compared to DNA extracted from fresh tissue. Conclusions: Our protocol could also be generalized to capture long sequences from specific nuclear fragments. | en_US |
| dc.faculty | Ciencias Exactas y Naturales | es |
| dc.id.author | 1711964690 | |
| dc.id.author | 1711059137 | |
| dc.id.type | 1 | |
| dc.identifier.doi | https://doi.org/10.1002/aps3.1243 | |
| dc.identifier.issn | 2168-0450 | |
| dc.identifier.issn | 2168-0450 | |
| dc.identifier.uri | https://repositorio.puce.edu.ec/handle/123456789/4927 | |
| dc.identifier.uri | https://bsapubs.onlinelibrary.wiley.com/doi/10.1002/aps3.1243 | |
| dc.indexed.database | Scimago Journal Rank | es |
| dc.language.iso | en | |
| dc.list.authors | Bethune, K., Mariac, C., Couderc, M., Scarcelli, N., Santoni, S., Ardisson, M., Martin, J. F., Montúfar, R., Klein, V., Sabot, F., Vigouroux, Y., & Couvreur, T. | |
| dc.magazine.pageRange | e1243 | |
| dc.magazine.title | Applications in Plant Sciences | en_US |
| dc.magazine.volumeChapter | 7(5) | |
| dc.rights | OpenAccess | en |
| dc.state | published | en_US |
| dc.subject | Biodiversidad | es |
| dc.subject | Filogenética | es |
| dc.subject | Hibridación de ADN | es |
| dc.subject | Biodiversidad | |
| dc.subject | Filogenética | |
| dc.subject | Hibridación de ADN | |
| dc.title | Long-fragment targeted capture for long-read sequencing of plastomes | en_US |
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