Biosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinnonii E5202H

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dc.careerCiencias Biológicasen_US
dc.category.authorprincipalen_US
dc.contributor.authorNarváez Trujillo, Elizabeth Alexandra
dc.contributor.correspondingStrobel, Scott A.en_US
dc.countryEcuadoren_US
dc.date.accessioned2023-11-04T21:42:31Z
dc.date.available2023-11-04T21:42:31Z
dc.date.issued15/02/2015
dc.dedication.authorTCen_US
dc.description.abstractAn endophytic fungus was isolated that produces a series of volatile natural products, including terpenes and odd chain polyenes. Phylogenetic analysis of the isolate using five loci suggests that it is closely related to Nigrograna mackinnonii CBS 674.75. The main component of the polyene series was purified and identified as (3E,5E,7E)-nona-1,3,5,7-tetraene (NTE), a novel natural product. Nonoxygenated hydrocarbons of this chain length are uncommon and desirable as gasoline-surrogate biofuels. The biosynthetic pathway for NTE production was explored using metabolic labeling and gas chromatography time of flight mass spectometer (GCMS). Two-carbon incorporation 13C acetate suggests that it is derived from a polyketide synthase (PKS) followed by decarboxylation. There are several known mechanisms for such decarboxylation, though none have been discovered in fungi. Towards identifying the PKS responsible for the production of NTE, the genome of N. mackinnonii E5202H (ATCC SD-6839) was sequenced and assembled. Of the 32 PKSs present in the genome, 17 are predicted to contain sufficient domains for the production of NTE. These results exemplify the capacity of endophytic fungi to produce novel natural products that may have many uses, such as biologically derived fuels and commodity chemicals.en_US
dc.facultyCiencias Exactas y Naturalesen_US
dc.id.author1000620672en_US
dc.id.type1en_US
dc.identifier.doi10.1007/s00253-014-6206-5en_US
dc.identifier.issn1432-0614
dc.identifier.urihttp://link.springer.com/article/10.1007/s00253-014-6206-5en_US
dc.identifier.urihttps://repositorio.puce.edu.ec/handle/123456789/5936
dc.indexed.databaseScimago Journal Ranken_US
dc.list.authorsLehr, N., Dunican, B., Orellana, E., Narváez, A., Strobel, S., et al.en_US
dc.magazine.pageRange3715–3728en_US
dc.magazine.titleApplied Microbiology and Biotechnologyen_US
dc.magazine.volumeChapter99en_US
dc.rightsClosedAccessen_US
dc.statepublisheden_US
dc.subjectBiosíntesisen_US
dc.subjectHidrocarburosen_US
dc.subjectEndófitasen_US
dc.subjectBiocarburanteen_US
dc.titleBiosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinnonii E5202Hen_US
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